Yuav Ua Li Cas Xaiv Qhov Zoo Fluorescence Microscope
Jun 08, 2023
Hauv kev sim txhua hnub, peb feem ntau siv lub tshuab ntsuas fluorescence. Yog li yuav ua li cas xaiv ib lub tshuab fluorescence uas tsim nyog yog qhov tseem ceeb heev.
Fluorescence microscopy kuj yog ib hom ntawm optical microscope, nrog rau qhov sib txawv tseem ceeb yog qhov sib txawv excitation wavelengths ntawm ob. Qhov no txiav txim siab qhov sib txawv ntawm cov qauv thiab kev siv ntawm fluorescent microscopes thiab cov khoom siv tshuaj lom neeg zoo tib yam.
Txhawm rau xaiv qhov tsim nyog fluorescence microscope, muaj ntau yam los xav txog.
1. Xav txog qhov excitation thiab emission spectra ntawm fluorophores koj yuav siv. Lub microscope yuav tsum muaj cov ntxaij lim dej uas tsim nyog thiab kev ua kom lub zog ua kom haum rau qhov zoo siab / emission spectra ntawm koj cov fluorophores.
Cov lim dej fluorescent tshaj plaws hauv kev ua lag luam yog Xiav, Ntsuab, Violet thiab Ultraviolet fluorescence excitation lim.
2.Nws muaj 2 hom illuminations rau fluorescence microscope: mercury teeb lossis LED teeb. Cov neeg siv tuaj yeem xaiv lub teeb raws li lawv cov kev xav tau.
3. Xav txog daim ntawv thov thiab hom qauv uas koj yuav ua duab. Rau cov qauv tuab, koj yuav xav tau lub tshuab tsom iav los yog multiphoton los txo cov fluorescence tom qab. Rau kev yees duab nyob hauv cell, koj yuav xav tau lub tshuab tsom iav nrog rau theem cua sov thiab ib puag ncig chamber.
4. Qee zaum, cov neeg siv xav thaij duab lossis duab. Lub koob yees duab digital tshwj xeeb rau fluorescence microscopy tuaj yeem daws qhov teeb meem no.Lub koob yees duab digital yuav tsum muaj qhov rhiab heev thiab tsis tshua muaj suab nrov los ntes cov teeb liab tsis muaj zog fluorescence. Lub koob yees duab kuj yuav tsum muaj tus ncej siab thiab daws teeb meem los ntes cov xwm txheej ceev ceev thiab cov duab zoo.
